4009 bc Search Results


mouse myeloma cells  (R&D Systems)
93
R&D Systems mouse myeloma cells
Mouse Myeloma Cells, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/4009+bc/10__1097_slash_nen__0b013e3181ce9f67-71-98-102?v=R%26D+Systems
Average 93 stars, based on 1 article reviews
mouse myeloma cells - by Bioz Stars, 2026-07
93/100 stars
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human recombinant brevican  (R&D Systems)
94
R&D Systems human recombinant brevican
FIGURE 4. Silencing of matrix metalloproteinase 19 (MMP19) reduces glioblastoma (GB) cell invasion in Transwell migration assays and into brain tissue sections. (A) U343 GB cells were transfected with no (control), control siRNA (C-Si), a nonrelated siRNA (C-n.r.; 250 Kmol/L siRNA against seprase, a serine protease), or with different concentrations MMP19-specific siRNA (Si-MP19). The MMP19 mRNA reduction was determined by quantitative reverse transcription polymerase chain reaction (calculated by the $$ CT method, n = 3 T SD). For subsequent assays, 250-Kmol/L transfected cells that showed MMP19 reduction to 17% were used. (B) In Boyden chamber assays through 5-Hm porous membranes, silenced and control cells migrated at similar rates when the chambers were uncoated (none); MMP19-silenced cells migrated more slowly through laminin-coated and somewhat more slowly through Matrigel- or <t>brevican-coated</t> membranes after 90 minutes (n = 15 for controls and Matrigel, n = 5 for others; mean T SD; *p G 0.05, ***p G 0.001); control siRNA-transfected cells behaved similar to control cells (not shown). (C) Invasion of MMP19-silenced U343 GB cells into sections of brain tissue is reduced compared with most controls. Transfected GB cells were either green (MMP19 siRNA) or red (control siRNA) fluorescence labeled; mixtures of equal numbers of both cells were applied on brain tissue slices. At 48 or 72 hours, the sections were fixed and inspected with an inverse microscope with apotome from the bottom (example: middle); red and green fluorescent cells were counted in the same areas and depths. Cell counts from 4 different experiments with individual cells/tissues are shown (right).
Human Recombinant Brevican, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/4009+bc/10__1097_slash_nen__0b013e3181ce9f67-71-62-102?v=R%26D+Systems
Average 94 stars, based on 1 article reviews
human recombinant brevican - by Bioz Stars, 2026-07
94/100 stars
  Buy from Supplier

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FIGURE 4. Silencing of matrix metalloproteinase 19 (MMP19) reduces glioblastoma (GB) cell invasion in Transwell migration assays and into brain tissue sections. (A) U343 GB cells were transfected with no (control), control siRNA (C-Si), a nonrelated siRNA (C-n.r.; 250 Kmol/L siRNA against seprase, a serine protease), or with different concentrations MMP19-specific siRNA (Si-MP19). The MMP19 mRNA reduction was determined by quantitative reverse transcription polymerase chain reaction (calculated by the $$ CT method, n = 3 T SD). For subsequent assays, 250-Kmol/L transfected cells that showed MMP19 reduction to 17% were used. (B) In Boyden chamber assays through 5-Hm porous membranes, silenced and control cells migrated at similar rates when the chambers were uncoated (none); MMP19-silenced cells migrated more slowly through laminin-coated and somewhat more slowly through Matrigel- or brevican-coated membranes after 90 minutes (n = 15 for controls and Matrigel, n = 5 for others; mean T SD; *p G 0.05, ***p G 0.001); control siRNA-transfected cells behaved similar to control cells (not shown). (C) Invasion of MMP19-silenced U343 GB cells into sections of brain tissue is reduced compared with most controls. Transfected GB cells were either green (MMP19 siRNA) or red (control siRNA) fluorescence labeled; mixtures of equal numbers of both cells were applied on brain tissue slices. At 48 or 72 hours, the sections were fixed and inspected with an inverse microscope with apotome from the bottom (example: middle); red and green fluorescent cells were counted in the same areas and depths. Cell counts from 4 different experiments with individual cells/tissues are shown (right).

Journal: Journal of Neuropathology & Experimental Neurology

Article Title: Matrix Metalloproteinase-19 is Highly Expressed in Astroglial Tumors and Promotes Invasion of Glioma Cells

doi: 10.1097/nen.0b013e3181ce9f67

Figure Lengend Snippet: FIGURE 4. Silencing of matrix metalloproteinase 19 (MMP19) reduces glioblastoma (GB) cell invasion in Transwell migration assays and into brain tissue sections. (A) U343 GB cells were transfected with no (control), control siRNA (C-Si), a nonrelated siRNA (C-n.r.; 250 Kmol/L siRNA against seprase, a serine protease), or with different concentrations MMP19-specific siRNA (Si-MP19). The MMP19 mRNA reduction was determined by quantitative reverse transcription polymerase chain reaction (calculated by the $$ CT method, n = 3 T SD). For subsequent assays, 250-Kmol/L transfected cells that showed MMP19 reduction to 17% were used. (B) In Boyden chamber assays through 5-Hm porous membranes, silenced and control cells migrated at similar rates when the chambers were uncoated (none); MMP19-silenced cells migrated more slowly through laminin-coated and somewhat more slowly through Matrigel- or brevican-coated membranes after 90 minutes (n = 15 for controls and Matrigel, n = 5 for others; mean T SD; *p G 0.05, ***p G 0.001); control siRNA-transfected cells behaved similar to control cells (not shown). (C) Invasion of MMP19-silenced U343 GB cells into sections of brain tissue is reduced compared with most controls. Transfected GB cells were either green (MMP19 siRNA) or red (control siRNA) fluorescence labeled; mixtures of equal numbers of both cells were applied on brain tissue slices. At 48 or 72 hours, the sections were fixed and inspected with an inverse microscope with apotome from the bottom (example: middle); red and green fluorescent cells were counted in the same areas and depths. Cell counts from 4 different experiments with individual cells/tissues are shown (right).

Article Snippet: Cell migration was monitored as described ([21] usually after 90 minutes) in 48-well Boyden-chambers (Neuro Probe, Baltimore, MD) using polycarbonate membranes with 5-Km pores that were uncoated (basal migration) or coated with Matrigel (a basement membrane protein mixture secreted by EHS mouse sarcoma cells containing laminin, collagen IV, heparan sulfate proteoglycans, and entactin), laminin (both from BD Biosciences, San Jose, CA), or human recombinant brevican (a member of the lectican family of chondroitin sulfate proteoglycans that is predominantly expressed in the CNS and is a major brain ECM component), and human recombinant glycosylated Asp23-Pro911, C-terminal His-tagged expressed in mouse myeloma cells (4009-BC; R&D Systems, Minneapolis, MN).

Techniques: Migration, Transfection, Control, Reverse Transcription, Polymerase Chain Reaction, Labeling, Microscopy

FIGURE 5. Matrix metalloproteinase 19 (MMP19) degrades brevican. Human recombinant brevican (1, 1 Kg; 2 and 3, 3 Kg) was incubated at 37-C at pH 7.4 with no MMP19 (1, control), MMP19 inactive mutant (2, MMP19EA), or wild-type MMP19 (3, MMP19 WT). Aliquots were withdrawn after different times (0, 10, 24, 34 hours) and examined by immunoblotting. The intensity of the 160-kd brevican band diminishes, and several fragments appear in a time-dependent manner in the MMP19WT lanes.

Journal: Journal of Neuropathology & Experimental Neurology

Article Title: Matrix Metalloproteinase-19 is Highly Expressed in Astroglial Tumors and Promotes Invasion of Glioma Cells

doi: 10.1097/nen.0b013e3181ce9f67

Figure Lengend Snippet: FIGURE 5. Matrix metalloproteinase 19 (MMP19) degrades brevican. Human recombinant brevican (1, 1 Kg; 2 and 3, 3 Kg) was incubated at 37-C at pH 7.4 with no MMP19 (1, control), MMP19 inactive mutant (2, MMP19EA), or wild-type MMP19 (3, MMP19 WT). Aliquots were withdrawn after different times (0, 10, 24, 34 hours) and examined by immunoblotting. The intensity of the 160-kd brevican band diminishes, and several fragments appear in a time-dependent manner in the MMP19WT lanes.

Article Snippet: Cell migration was monitored as described ([21] usually after 90 minutes) in 48-well Boyden-chambers (Neuro Probe, Baltimore, MD) using polycarbonate membranes with 5-Km pores that were uncoated (basal migration) or coated with Matrigel (a basement membrane protein mixture secreted by EHS mouse sarcoma cells containing laminin, collagen IV, heparan sulfate proteoglycans, and entactin), laminin (both from BD Biosciences, San Jose, CA), or human recombinant brevican (a member of the lectican family of chondroitin sulfate proteoglycans that is predominantly expressed in the CNS and is a major brain ECM component), and human recombinant glycosylated Asp23-Pro911, C-terminal His-tagged expressed in mouse myeloma cells (4009-BC; R&D Systems, Minneapolis, MN).

Techniques: Recombinant, Incubation, Control, Mutagenesis, Western Blot